MALDI-MSI of Lipids in Human Skin



Fig. 1
MALDI-MS images of a cross section of untreated human skin (with the stratum corneum at the top of each image), acquired at a spatial resolution of 30 μm × 30 μm. Images labeled (b) (m/z 760) and (e) (m/z 939) have been normalized against the matrix peak, m/z 417 (c), and the images labeled (a) (m/z 760) and (d) (m/z 939) have not been normalized [18]





 






    Acknowledgments

    This work was supported by the Biomolecular Sciences Research Centre of Sheffield Hallam University.


    References



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    Enthaler B, Pruns JK, Wessel S, Rapp C, Fischer M, Wittern KP (2012) Improved sample preparation for MALDI–MSI of endogenous compounds in skin tissue sections and mapping of exogenous active compounds subsequent to ex-vivo skin penetration. Anal Bioanal Chem 402(3):1159–1167CrossRefPubMed


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    Sahle FF, Gebre-Mariam T, Dobner B, Wohlrab J, Neubert RHH (2015) Skin diseases associated with the depletion of stratum corneum lipids and stratum corneum lipid substitution therapy. Skin Pharmacol Physiol 28:42–55CrossRefPubMed


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    Roy S, Touboul D, Brunelle A, Germain DP, Prognon P, Laprévote O, Chaminade P (2006) Imaging mass spectrometry: a new tool for the analysis of skin biopsy. Application in Fabry’s disease. Ann Pharm Fr 64(5):328–334CrossRefPubMed

    Nov 11, 2017 | Posted by in GENERAL RADIOLOGY | Comments Off on MALDI-MSI of Lipids in Human Skin

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