Fig. 1
TOF-SIMS ion images of a complete sagittal rat brain section (22.4 × 22.4 mm2). (a–c) TOF-SIMS ion images in the positive ion mode. (a) m/z 184.1 (phosphocholine head group); (b) m/z 385.4 ([cholesterol-H]+); (c) m/z 796.8 ([PE 40:4 + H]+, [PE38:1 + Na]+, or [PC34:2 + K]+). (d–g) TOF-SIMS ion images in negative ion mode. (d) m/z 283.2 (fatty acid carboxylate [C18:0 − H]−); (e) m/z 429.3 ([vitamin E–H]−); (f) m/z 888.9 ([ST d18:1/24:1 − H]−); (g) three-color overlay between TOF-SIMS ion images C16:0 carboxylate ion (red), vitamin E (green), and [ST d18:1/18:0 − H]− (blue). For all TOF-SIMS images: primary ion Bi3 2+, 50 keV, 3.4 × 108 ions/cm2, area 22.4 × 22.4 mm2, 256 × 256 pixels, pixel size 87.5 × 87.5 μm2. The amplitude of the color scale corresponds to the maximum number of counts, mc, and could be read as [0, mc]. tc is the total number of counts recorded for the specified m/z (it is the sum of counts in all the pixels). (h) An optical ion image of the sagittal rat brain section. Adapted with permission from ref. [12]
Fig. 2
MALDI Mass Spectrometry Imaging of Lipids and Primary Metabolites on Rat Brain Sections
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TOF-SIMS ion images of a zoom-in sagittal rat brain section (500 × 500 μm2). (a) An optical picture of the sagittal rat brain section. Ion images were recorded in the square delimited in white. (b, c) TOF-SIMS ion images in the positive ion mode. (b) m/z 430.4 ([vitamin E]+). (c) m/z 796.7 ([PE 40:4 + H]+, [PE38:1 + Na]+, or [PC34:2 + K]+). (d–f) TOF-SIMS ion images in the negative ion mode. (d) m/z 255.2 (C16:0 carboxylate ion). (e) m/z 888.7 ([ST d18:1/24:1-H]−). (f) m/z 906.7 ([ST d18:1/24:0-OH-H]−). Primary ion Bi3 2+, 50 keV, 2.5 × 1011 ions/cm2, area 500 × 500 μm2, 256 × 256 pixels, pixel size 2 × 2 μm2. The amplitude of the color scale corresponds to the maximum number of counts, mc, and could be read as [0, mc]. tc is the total number of counts recorded for the specified m/z (it is the sum of counts in all the pixels). Adapted with permission from ref. [12]
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