DESI Mass Spectrometry Imaging (MSI)
Fig. 1 Parts of the Waters DESI sprayer 2.4 Software 1. Prosolia Omni Spray 2D: software to control the DESI source for DESI sprayer optimization (Prosolia Inc., Indianapolis, IN,…
Mass Spectrometry Imaging of Drugs of Abuse in Hair
Fig. 1 Cutting apparatus used for longitudinal sectioning of hair samples. (a) Image of the in-house built cutting apparatus and (b) schematic of the cutting apparatus 2. Following decontamination,…
Rodent Whole-Body Sectioning and MALDI Mass Spectrometry Imaging
Fig. 1. (a) Stereotaxic frame used to freeze the carcass while maintaining spinal alignment. (N.B. the array of holes in each side permits the free flow of the hexane/dry ice…
ToF-SIMS Parallel Imaging MS/MS of Lipid Species in Thin Tissue Sections
and Ron M. A. Heeren1 (1) Maastricht MultiModal Molecular Imaging (M4I) Institute, Maastricht University, Universiteitssingel 50, 6229, ER, Maastricht, The Netherlands (2) Physical Electronics, Inc., Chanhassen, Minnesota 55317, USA Anne Lisa Bruinen Email: a.bruinen@maastrichtuniversity.nl…
Droplet-Based Liquid Extraction for Spatially-Resolved Microproteomics Analysis of Tissue Sections
Fig. 1 Schematic representation of strategies used for microproteomics analysis using Liquid MicroJunction (LMJ) . The upper panel shows methods to define regions of interest (ROIs) according to highlighted regions,…
MALDI-MSI Analysis of Cytological Smears: The Study of Thyroid Cancer
Benign thyroid nodules Malignant thyroid nodules Follicular adenoma (FA) Conventional variant papillary thyroid carcinoma (cvPTC) Hyperplastic (Hp) Follicular variant papillary thyroid carcinoma (fvPTC) Hashimoto’s thyroiditis (HT) Uncertain malignant potential (UMP)…
MALDI-MS Imaging in the Study of Glomerulonephritis
Fig. 1 Sample stocking procedure for ITO glass slides with mounted tissue 3.1.2 Tissue Preparation for MALDI-MSI 1. Following removal from the freezer, the ITO glass slides with mounted…
Peptide Imaging: Maximizing Peptide Yield, Optimization of the “Peptide Mass Fingerprint”
Fig. 1 Chemical structures of nonionic surfactants; (a) n-Octyl ß-d-glucopyranoside (OcGlu), (b) N-Octyl 1-thio-ß-d-glucopyranoside (OcThio), (c) N-Decyl ß-d-maltoside (DDM), (d) N-Octanoyl-N-methylglucamin (MEGA-8) Concentrations of OcGlu higher than 0.1% (up to…
The Future in Disease Models for Mass Spectrometry Imaging, Ethical Issues, and the Way Forward
© Springer Science+Business Media LLC 2017Laura M. Cole (ed.)Imaging Mass Spectrometry Methods in Molecular Biology161810.1007/978-1-4939-7051-3_16 16. The Future in Disease Models for Mass Spectrometry Imaging, Ethical Issues, and the Way Forward Rebecca E. Day1 and Ieva Palubeckaite1…
Fresh Frozen Versus Formalin-Fixed Paraffin Embedded for Mass Spectrometry Imaging
Fig. 1 Basic workflow of an imaging experiment with key focus areas; sample collection, sample preparation, choice of organic matrix , mode of application, and finally choice of mass spectrometer…
