Fig. 1. Electron energy loss spectrum (EELS) from DNA with zero-loss peak, plasmon peak, and superpositioned elemental edges which originate from excitation of inner-shell or core levels of phosphorus, carbon,…
Fig. 1. A magnified view of a typical aluminum-coated NSOM probe fabricated using the heating and pulling method. A small spot of light can be seen emerging from the distal…
Fig. 1. Schematic illustration of a FIB/SEM system. The scheme shows a sample hit by both ions (Ga+) and electrons (e−) during FIB/SEM operation. 3 Methods 3.1 Preparation of…
Fig. 1. Schematic representation of XM-2, a soft X-ray microscope for nanoimaging biological cells (located at the Advanced Light Source, Lawrence Berkeley National Laboratory, Berkeley, California). This is the first…
Fig. 1. Verification for labeling of the cytoplasmic mRNA. (a) A DNA construct for the expression of the MS2-EGFP protein with an NLS. (b) DNA constructs for the expression of…
Fig. 1. Preparing Quetol mounting “bullets” in flat bottom embedding capsules. 3.6 Mounting the Cells for Sectioning 1. Write the name of the sample on the hardened Quetol resin…
Fig. 1. TEM image of negatively stained amyloid fibrils. Fibrils with three distinct morphologies (numbered 1, 2, and 3 ) are present in the image. 3.2.2 STEM Imaging of Unstained…
Fig. 1. The principle of uPAINT is based on the real-time imaging and tracking of single fluorescent ligands while they label their membrane biomolecules targets. A low concentration of fluorescent…
Fig. 1. Our combined confocal-pTIRFM-SPM microscope comprising an Olympus IX-70 inverted microscope, an Olympus Fluoview 500 confocal microscope, a home-built 3-line TIRF a Digital Instruments Bioscope scanning probe microscope and…
Fig. 1. A simplified diagram of the perfusion apparatus as described in Subheadings 2.1 and 3.1. 5. Krebs-Ringer Carbicarb buffer (KRC): 2.0 mM CaCl2, 11.0 mM d-glucose, 4.7 mM…
