Fig. 1. (a) Flow diagram of a typical immuno-EM protocol using the Tokuyasu technique. (b) Example of Tokuyasu immunolabeling. Scale bar 0.5 μm. Reproduced with permission from ref. 4. The…
Fig. 1. AFM tip functionalization with Fc fragments via NHS–PEG–Aldehyde linker. (i) Aminofunctionalization of silicon nitride (Si3N4) AFM tips via silanization with 3-aminopropyltriethoxysilane (APTES) in gas phase. (ii) Use of…
Fig. 1. Overview of the modes of operation of a 4Pi microscope. The effective point spread functions are characterized by their full width at half maximum (FWHM) and the relative…
Fig. 1. Schematic of a typical TIRF microscope (system 2) and representative images of a cluster of three β-cells under epifluorescent (left) and TIRF (right) illumination. TIRF microscopy employs an…
Fig. 1. Photophysical states involved in photoswitching. (a) The structure and absorption spectra of mEosFPthermo before (left) and after (right ) activation. UV light induces an intramolecular reaction in which…
Fig. 1. Principle of the reconstitution of membrane proteins by direct incorporation within supported lipid bilayer. (a) Direct incorporation of membrane proteins in SLB. (1) Incubation of solubilized membrane proteins…
Fig. 1. Basic principle of STED microscopy. STED microscopy is based on fluorescence quenching by stimulated emission. (a) Jablonski diagram of the molecular events during fluorescence. Absorption of a blue…
Fig. 1. Overview scheme of steps involved in cryo electron tomographic imaging of cells. 2 Materials Several pieces of equipment are required for performing cryo electron microscopy on cells. The…
The centers of the PSFs can also be determined by fitting them to 2D Gaussian distributions using a least-squares approach. This is computationally more intensive, and a number of solutions…
Fig. 1. Mass spectra acquired with ∼11,000 MRP and 75 μm-width slits in front of the detectors. The analyzed cell was isotopically enriched in 13C and 15N. The ions of…
