Fig. 1.1 Jablonski diagram Both excitation and emission are observable by means of a spectrofluorometer. Within this equipment the sample is exposed to a light source emitting a constant photon…

Fig. 28.1 Metabolic pathway of 5-ALA Fig. 28.2 Fluorescence spectrum of PpIX excited with 405 nm Figure 28.3 shows fluorescence images of various colorectal cancer cell lines (HT-29, CACO-2, DLD-1,…

Human pancreatic cancer cell lines In vitro + −  Mia Paca-2 x  FG x  BxPC-3 x  CFPAC x  Panc-1 x  Capan-1 x  XPA-1  x XPA-3  x XPA-4  x In vivo…

Fig. 27.1 ICG solution injection Fig. 27.2 Photodynamic eye (PDE) camera (Hamamatsu Photonics, Hamamatsu, Japan) Fig. 27.3 Lymphatic drain real-time visualization Fig. 27.4 Fluorescent lymph node visualization (permission by Kobe…

Fig. 37.1 The Fluostick™, optical head and control box We have seen previously that, in order to reach an accurate fluorescence performance, the whole optical acquisition chain must be optimized…

Fig. 11.1 Fluobeam® medical device; central processing unit and the camera head Fluobeam® Medical Device The Fluobeam® medical device allows visualizing, on a computer screen, the flow, the distribution and/or…

Fig. 36.1 Structures of glutaryl phenylalanine hydroxylmethyl rhodamine green (gPhe-HMRG). The gPhe-HMRG probe is activated with chymotrypsin secreted in pancreatic juice and emits HMRG fluorescence Figure 36.2 shows the trends…

Fig. 26.1 Schematic image of sentinel lymph node (SLN) concept. SLN has direct flow from the tumor and the first metastasis via lymph channel should occur from here. If SLN…

Fig. 33.1 Heart exposure and ligation of second diagonal artery We hypothesize that fluorescein will provide a detailed anatomic demarcation of the ischemic myocardium and help us document tissue recovery…

Fig. 18.1 PDE imaging system Fig. 18.2 Fluorescent images obtained using the PDE system. (a) White spots (possible bile leakage) on the cut surface of the liver. (b) Minor leakage…