Super-Resolution Imaging by Localization Microscopy
The centers of the PSFs can also be determined by fitting them to 2D Gaussian distributions using a least-squares approach. This is computationally more intensive, and a number of solutions…
Secondary Ion Mass Spectrometry Imaging of Biological Membranes at High Spatial Resolution
Fig. 1. Mass spectra acquired with ∼11,000 MRP and 75 μm-width slits in front of the detectors. The analyzed cell was isotopically enriched in 13C and 15N. The ions of…
Preparation of Gold Nanocluster Bioconjugates for Electron Microscopy
Fig. 1. The four most common strategies for bioconjugation to gold nanoparticles. The tear-shaped cartoon represents the biomolecule to be linked to the gold nanoparticle. Only the important functional groups…
Fluorescence In Situ Hybridization Applications for Super-Resolution 3D Structured Illumination Microscopy
Fig. 1. Comparative analysis of RNA/DNA FISH visualized by wide-field fluorescence microscopy and 3D-SIM. Upper panel: RNA-FISH on a mouse C2C12 cell nucleus with biotin-labeled cDNA-probes against the noncoding Xist-RNA,…
Super-Resolution Fluorescence Imaging with Blink Microscopy
Fig. 1. An example of Blink Microscopy data from filamentous actin on a glass surface. Left : At the beginning, all fluorophores are in their ON state. This ensemble of…
Introduction: Nanoimaging Techniques in Biology
Alioscka A. Sousa and Michael J. Kruhlak (eds.)Methods in Molecular BiologyMethods and ProtocolsNanoimaging2013Methods and Protocols10.1007/978-1-62703-137-0_1© Springer Science+Business Media, LLC 2013 1. Introduction: Nanoimaging Techniques in Biology Alioscka A. Sousa1 and Michael J. Kruhlak2 (1) National Institute of Biomedical Imaging…
