Fig. 1. Schematic illustration of a FIB/SEM system. The scheme shows a sample hit by both ions (Ga+) and electrons (e−) during FIB/SEM operation. 3 Methods 3.1 Preparation of…
Fig. 1. Schematic representation of XM-2, a soft X-ray microscope for nanoimaging biological cells (located at the Advanced Light Source, Lawrence Berkeley National Laboratory, Berkeley, California). This is the first…
Fig. 1. Verification for labeling of the cytoplasmic mRNA. (a) A DNA construct for the expression of the MS2-EGFP protein with an NLS. (b) DNA constructs for the expression of…
Fig. 1. Preparing Quetol mounting “bullets” in flat bottom embedding capsules. 3.6 Mounting the Cells for Sectioning 1. Write the name of the sample on the hardened Quetol resin…
Fig. 1. TEM image of negatively stained amyloid fibrils. Fibrils with three distinct morphologies (numbered 1, 2, and 3 ) are present in the image. 3.2.2 STEM Imaging of Unstained…
Fig. 1. The principle of uPAINT is based on the real-time imaging and tracking of single fluorescent ligands while they label their membrane biomolecules targets. A low concentration of fluorescent…
Fig. 1. Our combined confocal-pTIRFM-SPM microscope comprising an Olympus IX-70 inverted microscope, an Olympus Fluoview 500 confocal microscope, a home-built 3-line TIRF a Digital Instruments Bioscope scanning probe microscope and…
Fig. 1. A simplified diagram of the perfusion apparatus as described in Subheadings 2.1 and 3.1. 5. Krebs-Ringer Carbicarb buffer (KRC): 2.0 mM CaCl2, 11.0 mM d-glucose, 4.7 mM…
Fig. 1. (a) Flow diagram of a typical immuno-EM protocol using the Tokuyasu technique. (b) Example of Tokuyasu immunolabeling. Scale bar 0.5 μm. Reproduced with permission from ref. 4. The…
Fig. 1. AFM tip functionalization with Fc fragments via NHS–PEG–Aldehyde linker. (i) Aminofunctionalization of silicon nitride (Si3N4) AFM tips via silanization with 3-aminopropyltriethoxysilane (APTES) in gas phase. (ii) Use of…
