Fig. 1 Example of an object (#1605) in each of the collected channels (channel 5 bright field, channel 1 Hoechst 33258 and channel 2 PNA probe) as well as a…

Fig. 1 The spectral layout of four different IFC systems we have successfully used to implement versions of our method. In all cases, the excitation lasers and approximate emission channel…

Fig. 1 Setting up the Inspire software. (a) Cell classifiers panel with the area lower limit and area upper limit for channel 1 bright field selected (red box). Intensity lower…

Fig. 1 Confocal micrographs of parasites stained with Fluo-4. Fluo-4 signal is redistributed to the parasite cytosol when the digestive vacuole is breached, resulting in an increased area of fluorescence….

Fig. 1 Glovebox used for sample deoxygenation. The apparatus is shown with the location of valves and gauges for the gas cylinders and vacuum pump indicated   6. 500 μl,…

Fig. 1 Primary cells and commonly used cell lines exhibit false-positive PI staining. Primary cells and cell lines were harvested and stained with propidium iodide and DRAQ5. Primary cells included…

Fig. 1 Representative fluorescent cell image. Image gallery for one monocyte (CD14+) depicting all channels of fluorescence used for the present method. Ch01: Bright-field; Ch04: CD14-PE-eFluor610; Ch06: SSC; Ch02: TNF-α-FITC;…

Fig. 1 (a) Types of files. The ImageStreamTM produces *.rif files, in which the uncompensated images are stored. A compensation matrix can be produced and stored in a *.ctm file….

Fig. 1 Dotplots from FACSAria II. THP1 cells treated with TRAIL, 100 ng/ml for 20 h. (a) FSC-W versus FSC-H, region P1 denotes singlet events. (b) FSC/SSC plot. (c) TMRE/Sytox…

Fig. 1 Initial processing of IFC MK panel files and merging of CD41+ events. (a) Determination of enucleated red blood cells versus nucleated cells by DRAQ5 signal intensity. This strategy…