Polyvinyl Alcohol Particles
Ajita Deodhar • John A. Kaufman Polyvinyl alcohol (PVA) is one of the oldest, particulate embolic materials providing inexpensive, permanent occlusion of blood vessels. It is a water-soluble, colorless synthetic…
Ultrafast Microfluidic Cellular Imaging by Optical Time-Stretch
Fig. 1 Development of time-stretch imaging in ultrafast microparticle and single-cell imaging in microfluidic flow. (a) An ultrafast microfluidic flow of water-suspended metal microspheres (sphere diameters ranging from 10 to…
Applications of Imaging Flow Cytometry for Microalgae
Fig. 1 Analysis of a natural seawater population. (a) Scatterplot of area vs. chlorophyll fluorescence as an example of a conventional cytometer output. Subpopulations are not readily distinguishable. (b) An…
Fluorescent In Situ Hybridization in Suspension by Imaging Flow Cytometry
Fig. 1 Setting up the Inspire software. (a) Cell classifiers panel with the area lower limit and area upper limit for channel 1 bright field selected (red box). Intensity lower…
The Analysis of Cell Cycle, Proliferation, and Asymmetric Cell Division by Imaging Flow Cytometry
Fig. 1 The spectral layout of four different IFC systems we have successfully used to implement versions of our method. In all cases, the excitation lasers and approximate emission channel…
Quantitation of Chromosome Damage by Imaging Flow Cytometry
Fig. 1 Example of an object (#1605) in each of the collected channels (channel 5 bright field, channel 1 Hoechst 33258 and channel 2 PNA probe) as well as a…
Screening for Drugs Against the Plasmodium falciparum Digestive Vacuole by Imaging Flow Cytometry
Fig. 1 Confocal micrographs of parasites stained with Fluo-4. Fluo-4 signal is redistributed to the parasite cytosol when the digestive vacuole is breached, resulting in an increased area of fluorescence….
Sickle Cell Imaging Flow Cytometry Assay (SIFCA)
Fig. 1 Glovebox used for sample deoxygenation. The apparatus is shown with the location of valves and gauges for the gas cylinders and vacuum pump indicated 6. 500 μl,…
Accurate Assessment of Cell Death by Imaging Flow Cytometry
Fig. 1 Primary cells and commonly used cell lines exhibit false-positive PI staining. Primary cells and cell lines were harvested and stained with propidium iodide and DRAQ5. Primary cells included…
Using Image-Based Flow Cytometry with a FISH-Based FlowRNA Assay to Simultaneously Detect Intracellular TNF-α Protein and mRNA in Monocytes Following LPS Stimulation
Fig. 1 Representative fluorescent cell image. Image gallery for one monocyte (CD14+) depicting all channels of fluorescence used for the present method. Ch01: Bright-field; Ch04: CD14-PE-eFluor610; Ch06: SSC; Ch02: TNF-α-FITC;…
