Fig. 1 Development of time-stretch imaging in ultrafast microparticle and single-cell imaging in microfluidic flow. (a) An ultrafast microfluidic flow of water-suspended metal microspheres (sphere diameters ranging from 10 to…
Fig. 1 Analysis of a natural seawater population. (a) Scatterplot of area vs. chlorophyll fluorescence as an example of a conventional cytometer output. Subpopulations are not readily distinguishable. (b) An…
Fig. 1 Example of an object (#1605) in each of the collected channels (channel 5 bright field, channel 1 Hoechst 33258 and channel 2 PNA probe) as well as a…
Fig. 1 The spectral layout of four different IFC systems we have successfully used to implement versions of our method. In all cases, the excitation lasers and approximate emission channel…
Fig. 1 Setting up the Inspire software. (a) Cell classifiers panel with the area lower limit and area upper limit for channel 1 bright field selected (red box). Intensity lower…
Fig. 1 Confocal micrographs of parasites stained with Fluo-4. Fluo-4 signal is redistributed to the parasite cytosol when the digestive vacuole is breached, resulting in an increased area of fluorescence….
Fig. 1 Glovebox used for sample deoxygenation. The apparatus is shown with the location of valves and gauges for the gas cylinders and vacuum pump indicated 6. 500 μl,…
Fig. 1 Primary cells and commonly used cell lines exhibit false-positive PI staining. Primary cells and cell lines were harvested and stained with propidium iodide and DRAQ5. Primary cells included…
Fig. 1 Representative fluorescent cell image. Image gallery for one monocyte (CD14+) depicting all channels of fluorescence used for the present method. Ch01: Bright-field; Ch04: CD14-PE-eFluor610; Ch06: SSC; Ch02: TNF-α-FITC;…
Fig. 1 (a) Types of files. The ImageStreamTM produces *.rif files, in which the uncompensated images are stored. A compensation matrix can be produced and stored in a *.ctm file….
