Temporal Heterogeneity in Apoptosis Determined by Imaging Flow Cytometry
Fig. 1 Dotplots from FACSAria II. THP1 cells treated with TRAIL, 100 ng/ml for 20 h. (a) FSC-W versus FSC-H, region P1 denotes singlet events. (b) FSC/SSC plot. (c) TMRE/Sytox…
Imaging Flow Cytometric Analysis of Primary Bone Marrow Megakaryocytes
Fig. 1 Initial processing of IFC MK panel files and merging of CD41+ events. (a) Determination of enucleated red blood cells versus nucleated cells by DRAQ5 signal intensity. This strategy…
Assessment of Granulocyte Subset Activation: New Information from Image-Based Flow Cytometry
Fig. 1 Granulocyte Gating. Using the entire cell population, focused cells are identified using a histogram of bright-field gradient RMS (a), followed by single cells that are identified using a…
Detection and Characterization of Rare Circulating Endothelial Cells by Imaging Flow Cytometry
Fig. 1 Gating strategy for enrichment of CECs during acquisition on an ImageStream MKII. Debris and large aggregates were eliminated with the R1 gate (a). The majority of CD146 PE-negative…
FlowCam: Quantification and Classification of Phytoplankton by Imaging Flow Cytometry
Fig. 1 FlowCam instrumentation. (a) Laboratory benchtop unit. (b) Portable unit Table 1 Recommended phytoplankton cell size ranges (cell diameter), objective, and optimal analysis volume for different FlowCam flow cells…
Using Image-Based Flow Cytometry to Assess Monocyte Oxidized LDL Phagocytosis Capacity
Fig. 1 Monocyte and phagocytosis gating. Using all collected events, single cells are identified using a dot plot of bright-field aspect ratio vs. bright-field area (a) followed by the identification…
Studying T Cells N-Glycosylation by Imaging Flow Cytometry
Fig. 1 Schematic representation of the analysis using an example of data acquired after staining for L-PHA and TCR. (a) Sequence of analysis steps. (1) A histogram for gradient RMS…
Analysis of Nucleocytoplasmic Protein Shuttling by Imaging Flow Cytometry
Fig. 1 The similarity histogram and a representative imagery panel of ARPE-19 cells non-stimulated (a, b) and stimulated with 10 ng/ml TNF-alpha (c, d). Ch2—Bright-field; Ch3—staining with NF-kappa Beta antibodies,…
Principles of Amnis Imaging Flow Cytometry
Fig. 1 Fluorescence detection limit and dynamic range as a function of cell size. Because each cell image covers multiple pixels, the sensitivity and dynamic range of cell measurements in…
Quantitative Functional Morphology by Imaging Flow Cytometry
Fig. 1 Size parameter in E. coli analysis by imaging flow cytometry. Examples of rods, roundish and intermediate-sized bacteria on images (panels) and on dotblot (area vs. aspect ratio intensity)…
