Fig. 1 Granulocyte Gating. Using the entire cell population, focused cells are identified using a histogram of bright-field gradient RMS (a), followed by single cells that are identified using a…
Fig. 1 Gating strategy for enrichment of CECs during acquisition on an ImageStream MKII. Debris and large aggregates were eliminated with the R1 gate (a). The majority of CD146 PE-negative…
Fig. 1 FlowCam instrumentation. (a) Laboratory benchtop unit. (b) Portable unit Table 1 Recommended phytoplankton cell size ranges (cell diameter), objective, and optimal analysis volume for different FlowCam flow cells…
Fig. 1 Monocyte and phagocytosis gating. Using all collected events, single cells are identified using a dot plot of bright-field aspect ratio vs. bright-field area (a) followed by the identification…
Fig. 1 Schematic representation of the analysis using an example of data acquired after staining for L-PHA and TCR. (a) Sequence of analysis steps. (1) A histogram for gradient RMS…
Fig. 1 The similarity histogram and a representative imagery panel of ARPE-19 cells non-stimulated (a, b) and stimulated with 10 ng/ml TNF-alpha (c, d). Ch2—Bright-field; Ch3—staining with NF-kappa Beta antibodies,…
Fig. 1 Fluorescence detection limit and dynamic range as a function of cell size. Because each cell image covers multiple pixels, the sensitivity and dynamic range of cell measurements in…
Fig. 1 Size parameter in E. coli analysis by imaging flow cytometry. Examples of rods, roundish and intermediate-sized bacteria on images (panels) and on dotblot (area vs. aspect ratio intensity)…
Fig. 4.1 Electron micrograph image of a myocardial capillary (Courtesy Clinical Science Journal, 2004) The Fig. 4.2 is the result of contrast limited adaptive histogram equalization (CLAHE) which is to adjust the…
Fig. 5.1 Original image (Exudative maculopathy) From observing Fig. 5.1 the Exudates are seen as yellow patches on the retina. Figure 5.2 is the resultant image of the proposed method. But here the…
